Imaging Biomarkers

Thomas L. Mindt is a well-recognized expert in the development of radiotracers with focus on radiometalls/peptides and one of our Key Researcher of the LBI AD leading the programme line on Imaging Biomarkers.

The program line Imaging Biomarkers is in charge of the development, production and preclinical evaluation of radiolabelled, tumour-targeting imaging probes (radiopharmaceuticals, radiotracers).

Radiopharmaceuticals of interest are conjugates that combine a radionuclide specific for a given application (Table 1) with a biological vector that is specific for a recognition element overexpressed by tumour cells (Figure 1). Depending on the application, the nature of the radionuclide, and the vector molecule, different chemical and biological techniques are required for their preparation.

Figure 1: Schematic sketch of the general design of tumour targeting radiotracers.

Table 1: Examples of radionuclides for different applications in nuclear medicine.

For preclinical evaluation, the radiopharmaceuticals are first tested in vitro (on cells) for their tumour-targeting properties, which usually includes experiments determining cellular uptake, affinity towards a given receptor/antigen, and other parameters (e.g., metabolic and enzymatic stability, hydrophilicity on the basis of physicochemical methods and new applied models, plasma protein binding, cell internalisation.). Promising candidates are then carried forwards to in vivo investigations (in mice) for determination of their pharmacokinetic and -dynamic properties employing biodistribution experiments and/or small animal imaging by ┬ÁSPECT/PET/CT.

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